Table 1.
Antibodies used for immunohistochemical staining
| Antigen | Distributor/catalog number | Dilution | Incubation | Visualization system |
|---|---|---|---|---|
| IL-2 | R&D/AF-502-NA | 2 μg/ml | 30 min, 37 °C | LSAB (Dako) |
| IL-4 | Santa Cruz/sc-53084 | 1:50 | 16 h, 4 °C | EnVision (Dako) |
| IL-6 | Abcam/ab-6672 | 1:1200 | 16 h, 4 °C | EnVision (Dako) |
| IL-10 | R&D/AF-519/NA | 5 μg/ml | 30 min, 37 °C | LSAB (Dako) |
| IFN-γ | R&D/AF-585-NA | 5 μg/ml | 30 min, 37 °C | LSAB (Dako) |
| TNF-α | Abcam/ab-1793 | 1:100 | 16 h, 4 °C | EnVision (Dako) |
| TGF-β1 | Santa Cruz/sc-52893 | 1:500 | 16 h, 4 °C | EnVision (Dako) |
| MMP-2 | Santa Cruz/sc-13595 | 1:50 | 16 h, 4 °C | EnVision (Dako) |
| MMP-9 | Abcam/ab-58803 | 1:100 | 16 h, 4 °C | LSAB (Dako) |