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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Mol Biosyst. 2014 Feb;10(2):196–200. doi: 10.1039/c3mb70471h

Fig. 5.

Fig. 5

FRET signals detected from the purified constitute proteasome (A–C) treated with FRET pairs: A. UKPCy3:LKSCy5 (left) and LKSCy3:UKPCy5 (right); B. LKSCy3:NC012Cy5 (left) and UKPCy3:NC012Cy5 (right). C. FRET signals were attenuated by co-treatment with an excess of non-fluorescent proteasome inhibitors (PI). RFU: Relative fluorescence units. D. FRET signals detected from RPMI multiple myeloma cell lysates treated with UKPCy3:LKSCy5 pair (Left). When UKPCy3 was co-incubated with LKSCy5 in RPMI8226 cell lysates, UKPCy3 selectively labeled β1i, whereas LKSCy5 selectively labeled β5.