Table 2.
Aromatic arginine stack interactions in DysF domains
| Stack | Dysferlin inner domain | W/R type | Interactions | Myoferlin inner domain equivalent (Residue mentioned in interaction) | Outer Dysferlin DysF equivalent |
|---|---|---|---|---|---|
| 1 |
R1048 |
Aliphatic W992 |
Planar interaction to Y1034 in crystal contact |
K1029 |
P1100 |
| |
|
|
H Bond to Q1010 in crystal contact |
|
|
| |
|
|
Interacts with crystallisation phosphate |
|
|
| |
|
|
Guanadinium Beyond NH/CZ of W992 |
|
|
| 1 |
W992 |
|
H bonds to crystallisation phosphate |
W973 |
W924 |
| 1 |
R1046 |
Perpendicular W992 |
NH points to 6 membered ring of W992 |
R1027 |
R1096 |
| |
|
|
H bonds to crystallisation phosphate |
|
|
| |
|
|
H bond to E951 |
(D392) |
(A879) ?E880 |
| 2 |
C988 |
|
H bond to 5 membered ring of W994 |
C969 |
P920 |
| |
W994 |
|
|
W975 |
W926 |
| |
R1044 |
Planar W994/ Aliphatic W999 |
Planar stack above 5 membered ring W994 |
R1025 |
R1096 |
| 2 |
W999 |
|
R1044 guanadinium is beyond NE/CZ edge |
W980 |
W930 |
| |
|
|
Aliphatic interaction with side chain of K983 |
|
|
| 2 |
K983 |
|
Just over 4 Å above 6 membered ring of W1042 |
P964 |
K915 |
| 2 |
W1042 |
|
H bond to E955 |
L1023 (Q936) |
W1094 (E883) |
| 3 |
R1040 |
Aliphatic W1012 |
H bond to E955 |
R1021 (Q396) |
R1092 (E883) |
| |
|
|
Guanadinium Beyond NE-CZ of W1012 |
|
|
| 3 |
W1012 |
|
|
W993 |
W1069 |
| 3 |
R1038 |
Planar W1012 |
Planar stack to 5 membered ring of W1012 |
R1019 |
R1090 |
| |
|
Planar W965 |
Planar stack to 5 membered ring of W965 |
|
|
| 3 |
W965 |
|
H bond to major conformation of E1031 |
W946 (E1012) |
W894 |
| 3 |
R959 |
Aliphatic W965 |
Guanadinum Beyond NE-CZ of W965 |
R940 |
K887 |
| |
|
|
H bonds to major and minor conformations of E1031 |
|
|
| Opposite Face |
F954 |
|
|
Y935 |
Y882 |
| |
R1039 |
Planar F954 |
Close to planar Stack above F954 |
R1020 |
R1090 |
| |
|
Planar W1027 |
H bond to E1013 |
(E994) |
(E1070) |
| |
|
|
Planar Stack above 5 membered ring of W1027 closer to NE than centre |
|
|
| W1027 | W1008 | Unclear |