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Comparison of native residue B-factors in stabilizing and destabilizing engineered disulfide bonds. The native structures associated with engineered disulfides previously reported as stabilizing (S) or destabilizing (D), based on experimental evidence, were analyzed with DbD2. The mean B-factor for residues involved in stabilizing disulfide bonds was 31.6 compared with 16.5 for those involved in destabilizing bonds, P = 0.066.
