Figure 6.

EBV infection promotes telomere uncapping. (a) Representative micrographs illustrating the presence of uncapped telomeres 2 weeks after EBV infection as assessed by the presence of telomere lacking colocalized TRF2. (b) Quantification of telomere localized TRF2 signals. The cells were stained by immunofluorescence-FISH (IF-FISH) and telomeres (red) and TRF2 (green) were identified. The intensity of green fluorescence overlapping with each red telomere was quantified using the MetaExpress software in approximately 2000 telomere signals. A compilation of the data recorded in three independent experiments is shown in the figure. (c) Western blot illustrating the expression of components of the shelterin and ATRX/DAXX complexes in EBV-infected or mitogen-induced blasts cultured for 14 days. Total cell lysates were fractioned by sodium dodecyl sulfate–polyacrylamide gel electrophoresis, and blots were probed with the indicated antibodies. (d) Densitometry quantification of protein expression. The relative intensity compared with the β-actin-specific band is shown. Mean±s.e. of five experiments.