Figure 5.

Association between CRKL and YES. (a) Decreased CRKL with doxycycline induction of shCRKL leads to a decrease in activated SFK (pY416) in both RD and Rh30 cell lines. Beta-actin served as loading control. (b) IP of three SFK, included FYN, SRC and YES, with mouse antibodies targeting the respective SFK from RD and Rh30 cell lines with or without CRKL knockdown. Rabbit anti-SRC or YES was used to probe the respective SFK IP blots and mouse anti-FYN was used for the FYN IP (lower panel). SFK IP blots were probed with mouse anti-phospho-tyrosine (upper panel). In both cell lines, FYN was weakly tyrosine phosphorylated compared with SRC and YES and only YES tyrosine phosphorylation was diminished with CRKL knockdown. Experiments in a and b were replicated at least twice.