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. 2013 Nov 8;456(Pt 2):205–217. doi: 10.1042/BJ20130591

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© 2013 The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

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(A) D/f plots of JP2^1–452 binding to DOPS containing SLBs with and without 1% PtdIns(3,4,5)P₃ (PIP3). (B) D/f plots for JP2 in the absence and presence of PtdIns(3,4,5)P₃ or PtdIns(4,5)P₂ (PIP2) compared with DOPS. (C) f/t plot of JP2 binding to DOPC/DOPE/POPC/PtdIns(3,4,5)P₃ (55:15:29:1, by vol.), arrows indicate addition of JP2. (D) D/f plot illustrating that the conformation of JP2 bound to PtdIns(3,4,5)P₃ is different than JP2 binding to either DOPS or DOPS plus PtdIns(3,4,5)P₃.