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. Author manuscript; available in PMC: 2014 Jan 22.
Published in final edited form as: Virology. 2003 Sep 30;314(2):601–616. doi: 10.1016/s0042-6822(03)00461-6

Fig. 6.

Fig. 6

NS3-epitope mapping of effector cells from chimpanzees 325 after challenge with 1-100 RNA (+) virion molecules. PBLs from chimpanzee 325 were tested for cellular response to pools of 10-mer NS3 overlapping peptides using IFN-γ ELISPOT assay as described under Materials and methods. PBLs at time 0 (A), and after challenge with an estimated one RNA (+) molecule (B), or after challenge with 10 (C), and 100 (D) RNA (+) molecules, were incubated with pools of ten 10-mer overlapping peptides of NS3 (HCV genotype 1b) at a concentration of 4 nM for each peptide. The numbers of IFN-γ-secreting cells (ISCs) were measured 18 h after in vitro stimulation. The numbers of HCV-specific ISCs/106 cells were plotted after subtracting the background (media alone). The cutoff value of ISCs (horizontal line) was calculated as described under Materials and methods and estimated to be approximately 217 ISCs/106.