Fig. 1.

Immunostaining with HIF 1α antibody, TUNEL, and DAPI in SHSY-5Y cells to investigate the effect of TM6008. Just after hypoxia, the numbers of TUNEL-positive and HIF-positive cells were higher than those in the control in cultures of SHSY-5Y cells. At 3 days after hypoxia, the number of HIF-positive cells cultured in the presence of TM6008 was further augmented, compared with the number of HIF-positive cells cultured in the absence of TM6008. Moreover, the number of HIF 1α-positive cells was significantly higher after 7 days of culture in the presence of TM6008. Notably, when HIF 1α was activated, it was translocated to the nucleus. Therefore, the white signal on the nucleus were shown. However, the number of TUNEL-positive cells was lower after 7 days of culture in the presence of TM6008, compared with the number of cells cultured in the absence of TM6008