Fig. 2.

(a), (b) The numbers of HIF-positive cells just after hypoxia and after 3 and 7 days of culture in the presence of TM6008 were larger than that in the control, whereas the number of TUNEL-positive cells just after hypoxia and after 3 and 7 days of culture in the presence of TM6008 were smaller than that in the control. (c) Relationship between cell death and the concentration of TM6008. Cell death was significantly reduced in the presence of 50 μM of TM6008, but was increased in the presence of more than 50 μM of TM6008, with a sharp increase observed in the presence of 200 μM of TM6008. The latter dose appeared to be toxic to the cells