Fig. 3.
Separation of polyprenyl phosphate oligomerhomologues by reversed-phase ion-pair high-performance liquid chromatography. Typical chromatograms of phosphorylated polyprenols (CiP) from fir needles using a reversed-phase Kromasil C18-column 250 × 4.0 mm inner diameter, 5 μm (Eka Chemicals, Sweden) and the mobile phase with the addition 0.020 M tetrabutylammonium dihydrogen phosphate. Conditions: (a) ethanol/hexane (87:13 v/v); (b) ethanol/hexane (90:10 v/v); flow rate: 0.85 ml/min (from Kozlov and Danilov 2012)