Fig. 3.

Knockdown of TMEM16 channels affects directionality differentially. a and b Micrographs of the wound healing assay at the beginning of the experiment and at t = 4 h. Migration into the wounded area is reflected by movement into the “y-direction” of a coordinate system. c Schematic representation of the analysis of directional migration. The directionality index is calculated as the ratio of net translocation D and total path length L. d and e Directional movement into the wound plotted as a function of time. f Comparison of the directionality indices of the different ELA cell clones. TMEM16A knockdown leads to an impaired directionality. Data is a representative of four or more independent experiments ± SEM with n ≥ 40 cells. Asterisk indicates P < 0.05