Table 1.
Analysis of cell migration in TMEM16A, 16F, and 16K KD clones
| Wt | Mock | miTMEM16A | miTMEM16F | miTMEM16K | |||||
|---|---|---|---|---|---|---|---|---|---|
| -5 | -7 | -9 | -10 | -15 | -16 | ||||
| Translocation (μm) | 83.0 ± 5.8 | 88.9 ± 4.5 | 89.7 ± 6.9 | 81.2 ± 5.4 | 66.6 ± 4.8 ** | 48.5 ± 2.7*** | 51.0 ± 3.0*** | 53.2 ± 3.2*** | 54.7 ± 1.2*** |
| Migratory speed (μm/min) | 0.48 ± 0.03 | 0.53 ± 0.03 | 0.61 ± 0.03 | 0.65 ± 0.03** | 0.44 ± 0.02** | 0.30 ± 0.01*** | 0.29 ± 0.01*** | 0.32 ± 0.01*** | 0.29 ± 0.02*** |
| Directional migration (μm) | 66.7 ± 5.2 | 72.1 ± 4.2 | 72.4 ± 6.7 | 64.4 ± 5.8 | 54.3 ± 4.5** | 36.4 ± 2.6*** | 40.3 ± 3.1*** | 34.8 ± 3.3*** | 43.5 ± 3.2*** |
| Directionality index | 0.72 ± 0.03 | 0.69 ± 0.02 | 0.59 ± 0.03* | 0.53 ± 0.03* | 0.62 ± 0.03* | 0.68 ± 0.03 | 0.71 ± 0.02 | 0.68 ± 0.02 | 0.76 ± 0.02* |
The asterisks denote statistically differences between mock and KD clones
*P < 0.05; **P < 0.01; ***P < 0.001, respectively