Figure 2.

Generation of transgenic lines with similar expression levels. A, schematic representation of the fusion peptides used to generate transgenic flies. Amino-acid single-letter code is used. Nec, the signal peptide of necrotic (MASKVSILLLLTVHLLAAQTFAQ); (H)₆ indicates the 6 × His-tag. The arrow indicates the site of cleavage in the secretory pathway. The rectangle encloses the sequence shared by Bri₂-23, ABri and ADan. B, agarose gels showing the PCR products obtained from genomic DNA of transgenic lines carrying one copy of untagged (arrowheads) or two copies: one untagged and the other with a 6 × His-tagged peptide DNAs (arrows). On the right, molecular markers in base-pairs (bp). C, similar levels of mRNA expression of Bri₂-23, ABri and ADan in the eye as determined by QRT-PCR normalized with tubulin mRNA. Bars represent the mean ± SEM of 3 independent experiments. A.U., arbitrary units.