Figure 3.

Amyloid peptides are properly processed in Drosophila eye. RIPA-soluble proteins extracted from flies expressing two copies of each peptide in the eye using the GMR-GAL4 driver. A, immunoreactivity with anti-His showing a very faint 3 kDa band consistent with HisBri₂-23 monomer (arrowhead). B, Western blot with 6E10 showing the 4.5 kDa monomeric Aβ42 in transgenic line (arrowhead). Synthetic (sp) Aβ42 was added to wild type Drosophila protein extract to compare molecular masses. C, immunoreactivity using anti-ABri revealed a specific band with the expected sizes of a monomer (arrowhead); on the right, synthetic (sp) ABri peptide added to the protein extract from a non-transgenic fly. D, Western blot using anti-ADan show the presence of a band (arrowhead) consistent with ADan monomer in the transgenic line. Wild type Drosophila protein extract was spiked with sp ADan peptide for comparison. M, monomers. CNT, control flies (GMR-GAL4/+). In all lanes, RIPA-soluble proteins from 15 head homogenates (150 μg) were loaded. Representative gels from more than 3 independent experiments for each genotype are shown.