Figure 8.
Histological analysis of fly brains. A, frontal sections of 21-day-old flies were stained with H&E and examined by bright-field microscopy. Vacuolization (>3 μm) of brain tissue was significantly higher in lines expressing Aβ42 as compared to Bri2-23, ABri and ADan. B, percentage of the area of tissue loss expressed as mean ± SEM. All lines expressed 2 copies of each peptide and 8–10 hemispheres per genotype were used for quantification. Asterisks indicate significant differences (One-way ANOVA, followed by Dunnett’s multiple comparison test). Scale bar = 50 μm.