Table 2.
Antibody | Host/clonality | Epitope retrieval | Blocking | Dilution/incubation | Negative control | Reaction system |
---|---|---|---|---|---|---|
Gd [32] |
Rabbit/polyclonal |
Not performed |
Reagent 1 (5 min) |
1: 800 (diluted in Dako antibody diluent)/o.n. (4°C) |
Rabbit pre-immune serum (Dako) |
Vectastain elite (rabbit IgG) kit (Burlingame, CA) |
GdA [26] | Mouse/monoclonal | Citrate buffer (5 min, pressure cooker) | 1.5% horse serum (20 min) | 1:2000 (diluted in Dako antibody diluent)/o.n. (4°C) | Mouse pre-immune serum (Dako) | Vectastain elite (mouse IgG) kit (Burlingame, CA) |