Figure 4. Clinical validation of micro-a-fluidic ELISA for CD4⁺ T lymphocyte count.

(a) and (b) Correlation of flow cytometry with micro-a-fluidic ELISA for CD4⁺ T lymphocyte count in 17 AIDS patient samples and 18 immunosuppressed patient samples, respectively. (c) and (d) Bland-Altman analysis for the difference between flow cytometry and micro-a-fluidic ELISA for CD4⁺ T lymphocyte count in 17 AIDS patient samples and 18 immunosuppressed patient samples, respectively. The 95% confidence interval on the mean is shown on the graphs. (e) Accuracy of CD4⁺ T lymphocyte micro-a-fluidic ELISA at the WHO clinical cutoff of 350 cells/μL for prioritizing ART. The samples were defined as positive once flow cytometry count was below 350 cells/μL, otherwise negative.