Figure 3.

Marginal Eye Field Cells Acquire AB Polarity in a Coordinated Manner

(A) Time-lapse movie of cells in the eye field of an embryo mosaically expressing Pard3-GFP. Cells are pseudocolored for clarity.

(B and C) High-resolution images of two marginal cells extracted from the movie in (A) as they polarize. Red arrows in (B) show Pard3-GFP punctae as they are transported apically. White arrows in (B) and (C) show apical accumulation of Pard3-GFP.

(D) Time-lapse movie of cells in the eye field of an embryo mosaically labeled with Kaede. Single cells were photoconverted from green to red by illumination with UV light. Four adjacent marginal cells are followed as they gradually coordinate their length.

(E) Unpolarized marginal cells are shorter than the polarized ones (black bar indicates mean [Mann-Whitney U test]; n = 30 from five embryos).

(F) Quantification of cell length of the four adjacent marginal cells in (D) over time (cell length is measured every 4.50 min).

(G) Schematic showing the experimental strategy.

White dotted lines (A) and (D) outline the eye field. Scale bars, 27 μm (A, B, and D). See also Figure S2 and Movie S3.