Figure 2.

Relative levels of apoptotic cells in Michigan Cancer Foundation (MCF-7) and MRC-5 treated with combination of drugs for different times. Cells incubated with the vehicle dimethyl sulfoxide (DMSO) were used as a control. (a and b) The percentage of apoptotic cells was measured using the AnnexinV FITC (FL1-H) and propidium iodide (PI) (FL2-H) assay. ^****P < 0.001 versus all other groups MCF-7 cells treated with combination of cholera toxin B and Salermide. ^***P < 0.05 versus all other groups MRC-5 cells. ^**P < 0.05 versus all other groups MCF-7 cells incubated with the DMSO was used as a control. ^*P < 0.05 versus 48 and 72 h groups MRC-5 cells incubated with the DMSO were used as control. (b) Cells that are AnnexinV-positive and propidium iodide negative are in early apoptosis as phosphatidyl serine (PS) translocation has occurred; although, the plasma membrane remains intact. Cells that are positive for both AnnexinV and PI either are in the late stages of apoptosis or are already dead as PS translocation has occurred and the loss of plasma membrane integrity is visible