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. Author manuscript; available in PMC: 2014 Jan 22.
Published in final edited form as: Mol Cell. 2011 May 20;42(4):489–499. doi: 10.1016/j.molcel.2011.04.015

Figure 3. Stress or PARP-13 overexpression alleviates miRNA-mediated repression.

Figure 3

(A) miRNA activity assay in untreated 293T cells or cells treated with 30 nM pateamine A (PatA), 1 μM hippuristanol (Hipp) or 250 μM arsenite (As) for 2 hr, where relative fold repression was measured as the activity of luciferase (upper panel) in the presence of the targeting siRNA normalized to a control siRNA; n = 3. (B) miRNA activity assay upon overexpression of SG-PARPs. Relative fold repression was measured as in panel A; n = 4. For panels A and B, error bars indicate SD; paired t-test p < 0.05 (*) and < 0.01 (**). (C) Antibodies against endogenous Ago2 was used for immunoprecipitation from cytoplasmic extract of HeLa cells treated with or without 250 μM arsenite for 30 min. On the right, the extract was pre-treated with 200 μg/ml RNase A for 20 min at 25°C. (D) Immunoprecipitates of GFP-tagged PARP-13.1 or -13.2 from cells treated with or without 20 nM pateamine A for 30 min were probed for pADPr, where cell extracts included 1 μM ADP-HPD. Asterisks indicate the position of the corresponding GFP-tagged PARPs. See also Figure S3.