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. Author manuscript; available in PMC: 2015 Feb 6.
Published in final edited form as: J Mol Biol. 2013 Nov 8;426(3):550–557. doi: 10.1016/j.jmb.2013.11.001

Figure 3. Microscopic analysis of cells with two ectopic tetO arrays and TetR-GFP, TetR-kGFP or TetR-tdTomato protein.

Figure 3

A. Quantitation of the microscopic analysis.

Percentages of cells with 1 focus and 2 foci are plotted for three strains, all of which have two tetO arrays located at HIS4 and LYS2 loci, plus one of the proteins: TetR-GFP, TetR-kGFP or TetR-tdTomato. Light grey bars – percentage of cells with 1 focus, dark grey bars – percentage of cells with 2 foci. p(1,2) = 3.45×10−5; p(1,3) = 7.3×10−4; p(2,3) = 3.38×10−3 (all p-values are < 0.01).

B. Representative images of cells.

In the case of cells with 2 foci, the difference in brightness of the two foci is due to the difference in the length of the two arrays (Figure 1A).