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. Author manuscript; available in PMC: 2015 Feb 6.
Published in final edited form as: J Mol Biol. 2013 Nov 8;426(3):550–557. doi: 10.1016/j.jmb.2013.11.001

Figure 5. Microscopic analysis of G2/M-arrested cells with tetO array at LYS2 locus and TetR-GFP or TetR-kGFP protein.

Figure 5

A. Quantitation of the microscopic analysis.

Percentages of cells with 1 focus and 2 foci are plotted for two strains, both of which have tetO array located at LYS2 locus, plus one of the proteins: TetR-GFP or TetR-kGFP. Cells were arrested in G2/M with nocodazole, so each cells contains two sister chromatids, each of which has tetO array at LYS2. Light grey bars – percentage of cells with 1 focus, dark grey bars – percentage of cells with 2 foci. p=2.22 x10−3< 0.01.

B. Representative images of cells.