Figure 7.

High Glucose Diet Suppresses Metformin-Induced Life Extension

(A) Metformin decreases lifespan on 0.25% d-glucose. See Figure S7A for 1% d-glucose.

(B) Metformin does not inhibit bacterial growth in the presence of 0.25% d-glucose.

(C) Scheme summarizing direct and indirect effects of metformin on the C. elegans/E. coli system. Dotted lines indicate hypothetical feedback loops.

Error bars represent SEM of at least three independent biological replicates. ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001. See also Figure S7. For statistics, see Table S7.