Figure S2.

Biguanides Inhibit Bacterial Growth, Related to Figure 2

(A and B) Metformin shortens lifespan in the absence of bacteria (bacterial deprivation on NGM plates) (Kaeberlein et al., 2006; Lee et al., 2006).

(C) Dose-dependent inhibition of E. coli OP50 growth by phenformin (LB liquid media, 18 hr period).

(D–E) Dose dependent inhibition of growth of Bacillus subtilis by metformin (LB liquid media, 18 hr period).

(F) Metformin causes altered bacterial lawn morphology in Bacillus subtilis.

(G) Inhibition of bacterial growth by metformin is more marked at pH = 6.0 than at pH = 7.0.

(H) Effects of metformin on C. elegans lifespan are pH-sensitive.

See Table S2 for statistics. Error bars, SEM. ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001.