Figure S3.

Effects of Metformin and Phenformin on Bacterial Growth and Lifespan of Various Bacterial Strains, Related to Figure 3

(A) Metformin shortens worm lifespan in the presence of the wild-type E. coli K-12 strain MG1655.

(B) E. coli sensitivity to growth inhibition by metformin shows no correlation with E. coli effects on wild-type worm lifespan.

(C) E. coli OP50-MR is resistant to growth inhibition by metformin compared to OP50 parent strain (c.f. Figure 2F).

(D) E. coli OP50-MR is resistant to growth inhibition by phenformin compared to OP50 parent strain.

(E) Relative resistance to growth inhibition by phenformin of E. coli OP50 (black) and OP50-MR (gray).

(F) OP50-MR increases lifespan in the absence of metformin.

For statistics see Table S3. Error bars, SEM. ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001.