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. 2013 Mar 25;32(47):5409–5420. doi: 10.1038/onc.2013.67

Figure 3.

Figure 3

BMP7 induces Snail and astrocytic differentiation. (a) Treatment with BMP7 for 2 and 24 h in several GBM cell lines; qRT–PCR analysis of Snail mRNA (left panel), and western blot analysis (right panel) of Snail, P-Smad1, Smad1 and α tubulin, which serves as loading control. (b) qRT–PCR analysis of Snail, GFAP, Nestin, Olig1 and Olig2 mRNA in U-3028 MG and U-3034 MG cells treated with BMP7 as indicated, and presented as average of two independent experiments. (c) Primary gliomasphere formation (>50 μm) upon BMP7 treatment of U-3028 MG and U-3034 MG cells for 6 days in stem cell medium. (d) qRT–PCR of Snail and GFAP mRNA in two independent cultures (samples) of human NPCs treated with BMP7 for 2 and 24 h, respectively.