Figure 6.

Snail decreases U-2987 MG tumorigenicity in vivo. Tumors derived after injecting adherent U-2987 MG cells (a, b) or cells dissociated from gliomaspheres (c–j). (a–f) Hematoxylin-eosin staining; (g–j) immunostaining with anti-GFP antibody. Panels g and h show the same section as panels e and f, respectively, and panels i and j are higher magnifications of panels g and h, respectively. Stars indicate tissue positions that are magnified 8 times. Panel b shows the absence of tumor development in the brain of a mouse engrafted with Snail-overexpressing cells. Panel d shows a section from an injected mouse brain that exhibited very rudimentary tumor growth. Panels e–j show sections from small but detectable tumors. Scale bar: 500 μm.