Fig. 4.

Kv subunits are not regulated by dorsal rhizotomy.

(A) A transverse section of the spinal cord with DRGs attached, illustrating the dorsal rhizotomy of the right central processes, stained for NF200, CGRP and IB4. Inset, GFAP staining illustrating astrocyte activation at the dorsal root entry zone of the injured side. (B) Bright field and polarized images of DRG sections from the contralateral (left) or ipsilateral (right) side of rhizotomized animals (7 days), subjected to ISH for Kv2.1 (top) or Kv2.2 (bottom) mRNA. (C) Percentage of neurons positive for Kv2 mRNA expression and quantification of signal intensity with or without dorsal rhizotomy (mean ± SEM, n = 3 animals/group, paired t-test for ipsilateral vs contralateral sides for each subunit, *p < 0.005). Scale bars = 50 μm.