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. 2013 Jul 22;32(50):5582–5592. doi: 10.1038/onc.2013.277

Figure 1.

Figure 1

Inhibition of FER induces cell spreading, actin stress fibre and FA formation. (a) Total cell lysates of the indicated breast cancer cell lines were prepared and resolved by SDS-polyacrylamide gel electrophoresis. FER kinase expression was analysed by immunoblot. (b) KD of FER in MDA-MB-231 cells using siRNA transfection. FER expression was analysed by immunoblot 72 h after transfection. Actin was used as a loading control. (c) Control and FER KD MDA-MB-231 cells were plated on collagen I-coated glass coverslips. The morphology of live cells was analysed by phase contrast microscopy (phase). F-actin (green) and phospho-paxillin (red) distribution was analysed in fixed cells by immunofluorescence (IF) microscopy. The percentage of cells showing stress fibers is shown in the right bar graphs. Scale bar=50 μm. The results shown are representative of three independent experiments.