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. 2013 Jul 22;32(50):5582–5592. doi: 10.1038/onc.2013.277

Figure 2.

Figure 2

FER regulates cell–matrix adhesion and integrin expression in breast cancer cells. (a) MDA-MB-231 FER KD cells were generated by transient transfection with the indicated siRNAs. FER KD cells were plated on 96-well plates coated with collagen I, fibronectin or laminin and the percentage of attached cells was determined. Values represent the relative proportion of attached cells ±s.e.m. * indicates significantly different proportions of adherent cells, relative to control (P<0.05, one-way analysis of variance (ANOVA)). (b) FER KD cells were trypsinized and surface expression of the indicated integrin subunits was analysed by fluorescence-activated cell sorting (FACS). Values represent mean fluorescence units ±s.e.m. * indicates significantly different integrin levels as compared with control (P<0.05, one-way ANOVA). The results shown are representative of three independent experiments.