Figure 4.

Activity of novel grafted peptides in vivo in experimental autoimmune encephalomyelitis in mice. (a) Clinical score of EAE mice after vaccination with MOG-grafted peptides (MOG3, dark blue line; MOG13, red line; MOG16, green line) and controls (kalata B1, light blue line; PBS, black line) was monitored. (b) The influence of MOG-grafted cyclotide vaccination on the formation of CNS inflammatory and demyelinating lesions was examined by histological studies of fixed tissue using hematoxylin/eosin, Luxol fast blue, and Bielshowsky silver staining. Regions of inflammation, demyelination, and axonal damage are highlighted by white arrows. (c) Proliferation of spleen cells in response to the encephalitogen MOG_35–55 and stimulation by the polyclonal activators, anti-CD3 and anti-CD28 antibodies. (d, e) Significantly reduced levels of the chemokine MIG (d) and TNFα (e) were demonstrated in non-stimulated spleen cell supernatants generated from animals treated with MOG3, MOG13, and kalata B1. * p < 0.05 compared to PBS control.