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. 2013 Dec 3;175(1):25–31. doi: 10.1111/cei.12198

Figure 1.

Figure 1

Flow cytometric analysis of T helper type 9 (Th9) cells percentage in atopic dermatitis (AD) patients, psoriasis patients and healthy controls. (a) Peripheral blood mononuclear cells (PBMCs) were isolated from a 16-year-old male AD patient (a1) and a 16-year-old male healthy individual (a2). These cells were stimulated with phorbol myristate acetate (PMA), ionomycin and GolgiStop for 4 h, stained for interleukin (IL)-9, interferon (IFN)-γ, IL-4 and IL-17A and analysed by flow cytometry. The numbers in the upper right quadrant represent the percentage of IL-9+ cells with expression of CD4+ cells in the gated population of CD4+ IFN-γ− IL-4− IL-17A− cells. (b) The percentage of Th9 cells in PBMCs was increased significantly in AD patients compared with psoriasis patients and healthy controls (*P < 0·01). Pso: psoriasis; HC: healthy controls.