Figure 3.

Poor recovery of natural killer (NK) T cells in untreated multiple myeloma (MM) patients after autologous stem cell transplant. Peripheral blood mononuclear cells (PBMCs) were isolated from blood collected from untreated MM patients prior to treatment (C1), after two cycles of lenalidomide induction (LEN) (C2), after three cycles of LEN induction (C3), at the end of induction (EoI), two maintenance cycles post-AuSCT (M2), four maintenance cycles post-autologous stem cell transplantation (ASCT) (M4) and six maintenance cycles post-ASCT (M6). (a) Representative fluorescence activated cell sorter (FACS) plots demonstrate how NKT cell subsets are analysed. (b) The frequency of T cells, NK cells and NKT cells were assessed at each time-point. CD4⁺ T cells and CD8⁺ T cells are expressed as a proportion of T cells. T cells, NK cells and NKT cells are expressed as a proportion of lymphocytes and of T cells as indicated. (c) T cells, NK cells and NKT cell for individual untreated MM patients are shown.