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. 2013 Dec 20;25(1):188–195. doi: 10.1021/bc400513z

Figure 3.

Figure 3

Silencing of the brain acid-soluble protein 1 gene (BASP1) by siRNA duplexes with fluorescent labels (F545) clicked to 3′-terminal 2′-O-(2-azidoethyl) anchors. (A) General organization (top) and labeling pattern of the siRNA duplex (bottom); for detailed RNA sequences see Table S1. (B) BASP1 siRNAs show cytoplasmic localization in DF1 cells visualized by fluorescence microscopy. The amounts of nucleofected siRNAs were 0.24 nmol. (C) Activities of 2′-az-F545 labeled BASP1 siRNAs and corresponding controls (random siRNA and unmodified siRNA) monitored by Northern analysis of BASP1 expression in DF1 cells. Expression of GAPDH served as loading control.