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. 2014 Jan 16;156(1-2):69–83. doi: 10.1016/j.cell.2013.12.008

Figure 2.

Figure 2

Organ-Specific Effects of Reduced Insulin Signaling on Tracheal Coverage

(A–C) Representative tracheation of the areas boxed in the cartoons in control larvae: body wall (A), midgut (anterior, B), and hindgut (mid-hindgut, C).

(D–F) Expression of PI3K-DN in tracheal terminal cells leads to reduced branching in body wall (D), midgut (anterior, E), and hindgut (mid-hindgut, F). For body wall: p = 0.001 (DSRF>PI3K-DN versus GAL4 control), p < 0.001 (DSRF>PI3K-DN versus UAS control), p = 0.03 (GAL4 versus UAS control), n = 13–15/set. For anterior midgut: p < 0.0001 (DSRF>PI3K-DN versus GAL4 control), p < 0.001 (DSRF>PI3K-DN versus UAS control), n = 15/set. For mid-hindgut: p < 0.0001 (DSRF>PI3K-DN versus GAL4 control), p < 0.0001 (DSRF>PI3K-DN versus UAS control), n = 19–25/set.

(G–I) Driving RNAi against InR from the same driver line does not affect body wall tracheae (G) but leads to reduced branching in the midgut (anterior, H) and hindgut (mid-hindgut, I).

Scale bars, 10 μm in all images except for (A), (D), and (G), 100 μm. For body wall: n = 15–20/set. For anterior midgut: p = 0.014 (DSRF>InR-RNAi versus GAL4 control), p = 0.014 (DSRF>InR-RNAi versus UAS control), n = 10/set. For mid-hindgut: p < 0.0001 (DSRF>InR-RNAi versus GAL4 control), p < 0.0001 (DSRF>InR-RNAi versus UAS control), n = 27–28/set. See also Figures S2 and S3.