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. 2014 Jan 13;28(1):81–93. doi: 10.1016/j.devcel.2013.12.001

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© 2014 The Authors

This is an open access article under the CC BY license (https://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

Augmin Is Essential for MT Generation around Chromatin

(A and B) Stills from movies of spindle reformation in embryos expressing Tubulin-GFP Histone-RFP (A) or EB1-GFP (B) injected with anti-Dgt6 antibodies.

(C) Stills from movies of D-HURP-GFP localization following cold treatment in an anti-Dgt6-injected embryo; localization of D-HURP-GFP is not dependent on Augmin.

(D) Composite kymographs of MT nucleation (EB1-GFP) during mitosis in control (WT) and anti-Dgt6-injected embryos following cold treatment; chromatin-dependent MT generation is completely absent upon Augmin disruption.

See also Movie S8. Scale bars, 5 μm.