Figure 6.

Centrosome/PCM Disruption Leads to Spindle Formation via Augmin-Dependent aMTOCs

(A) Stills from movies of spindle formation in an EB1-GFP-expressing cnn mutant embryo.

(B and C) Stills from movies of spindle formation in EB1-GFP expressing (B) and Tubulin-GFP; Histone-RFP embryos (C) injected with a high concentration of anti-DSpd-antibody. In all cases, spindles form predominantly from cytoplasmic, acentriolar MTOCs.

(D) Stills from movies of spindle formation in an EB1-GFP-expressing cnn embryo injected with anti-Dgt6 antibodies. MTs are present in interphase, but mitotic aMTOCs do not form.

Scale bars, 5 μm. See also Movie S9.