Figure 3.

Effects of ERK, p38, and AKT blockade on Col1 and Col3 expression. A) Representative Western blots showing Col1 and Col3 expression after CGS21680 stimulation on ERK1/2 and p38 blockade with U0126 (1 μM) and SB203580 (1 μM), respectively, and AKT blockade with either LY294002 (10 μM) or wortmannin (0.1 μM). B) Bands were quantified; data represent means ± sem of ≥3 independent experiments. Statistical analysis was performed by 2-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001 vs. control.