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Effect of ERK1/2 and AKT1/2/3 knockdown on collagen expression. A) Double siRNA ERK1/2 and triple siRNA AKT1/2/3 significantly reduce ERK1/2 and AKT expression. B) Effect of ERK1/2 and AKT1/2/3 knockdowns on Col1 and Col3 expression after CGS21680 incubation. Bands were quantified, and data represent means ± se of 4 independent experiments. ***P < 0.001 vs. control siRNA, Student's t test; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. control siRNA, 2-way ANOVA.
