Figure 8.

Divided progeny from PGE₂-pulsed cultures manifested higher pH2AX staining (indicative of DNA double-strand breaks) when cultures were treated with pan-caspase inhibitor, Z-VAD. CFSE-labeled B cells were activated in 96-well plates (10⁵ cells/200 μl), and activated cultures received PGE₂ (50 nM), as indicated, on d 2 and 4 and 40 μM Z-VAD (or DMSO vehicle) on d 4. On d 5, cultures were harvested, fixed, and stained intracellularly with PE-anti-pH2AX (or PE-IgG control). PE MFI was determined for cells whose light scatter and CFSE fluorescence represented the viable, divided subset prior to fixation. A) Numbers represent the RMFI (ratio of pH2AX MFI/MFI IgG control MFI). B) Bars represent the ratio of the RMFI in PGE₂-supplemented cultures/RMFI in nonsupplemented cultures of 2 experiments.