Table 2.
Frequency of codon 4 missense p53 gene mutations in TI-activated human B cells
| Mutated B cell | PGE2 pulse | Total | Silent | Missense | Location |
|---|---|---|---|---|---|
| T612, experiment 3 | |||||
| Cell A | No | 2 | 0 | 2 | Codon 56 (E→K); codon 82 (P→Q) |
| Cell B | Yes | 1 | 1 | 0 | |
| Cell C | Yes | 3 | 2 | 1 | Codon 96 (S→P)a |
| T597, experiment 6 | |||||
| B3-cell A | No | 1 | 1 | 0 | |
| B4-cell B | No | 1 | 1 | 0 | |
| B5-cell C | No | 1 | 0 | 1 | Codon 72 (R→C)b |
| D2-cell D | Yes | 1 | 0 | 1 | Codon 75 (P→L) |
| D5-cell E | Yes | 2 | 0 | 2 | Codon 89 (P→T); codon 96 (S→P)a |
a
The codon 96 S→P missense mutation was observed twice, in PGE2-pulsed cells of 2 distinct donors, from differing experiments. This mutation occurred in a MHT motif, which can be targeted during the secondary phase of AID-driven mutagenesis (65).
b
This mutation occurred in exon 4 codon 72, the site of a common functionally relevant SNP in humans. This cell reverted from expression of codon 72-Arg to codon 72-Cys.