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. Author manuscript; available in PMC: 2014 Jan 22.
Published in final edited form as: Stem Cells. 2011 Dec;29(12):1952–1962. doi: 10.1002/stem.748

Figure 6.

Figure 6

PTEN KD human embryonic stem cells (hESCs) generate teratomas containing all three germ layers with increased ectoderm and reduced endoderm potential. Control (A) and PTEN KD (B) teratoma sections were stained for MAP2 (green) and DAPI (blue) to show neuroectoderm regions. H & E stained control (C, E) and PTEN KD (D, F) teratoma sections showing gut (G), bone (B), and muscle (M) formation. Teratoma sections were immunostained for NESTIN (G) and MAP2 (H) and quantified using Ariol software (n = 4). Quantification of endoderm (I) and mesoderm (J) using H & E staining (for gut or bone and muscle) in control and PTEN KD teratoma sections (n = 6). Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; MAP2, microtubule-associated protein 2; PTEN KD, PTEN knockdown.