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. Author manuscript; available in PMC: 2014 Jan 22.
Published in final edited form as: Stem Cells. 2011 Dec;29(12):1952–1962. doi: 10.1002/stem.748

Figure 7.

Figure 7

PTEN KD human embryonic stem cells (hESCs) are more homogeneous and have increased pluripotency gene expression under self-renewing conditions. (A): FACS histogram of unstained control and PTEN KD hESCs cultured under self-renewing conditions showing total TRA-1-81-positive events. (B): The percentage of high TRA1-81-positive hESCs in control and PTEN KD hESCs. (C): FACS analysis of nuclear protein expression of OCT4, NANOG, and SOX2 in control and PTEN KD hESCs. Graph shows the percentage of TRA-1-81-positive hESCs that were positive for OCT4, NANOG, or SOX2. (D): Expression of pluripotency markers was evaluated using quantitative PCR (qPCR). (E): Expression of the early progenitor markers, PAX6 and GATA4, in control and PTEN KD hESCs. Graphs show PTEN KD fold over control. All experiments were performed using control and PTEN KD passage 37–45 hESCs. Error bars represent standard errors of three independent experiments and asterisks denote p value <.05. Abbreviations: FACS, fluorescence-activated cell sorting; PCR, polymerase chain reaction; PTEN KD, PTEN knockdown; UTF, undifferentiated embryonic cell transcription factor.