Figure S5.

Effect of Sik1 Knockdown on Period Length, Related to Figure 5

(A) Representative traces from Per2::Luc U2OS cells transfected with siSik1, siNT or siCry1. siSik1 (inducing > 90% knockdown of Sik1) induces period lengthening while siCry1 (>90% knockdown of Cry1) induces period shortening. Data summated in (B) ^∗∗∗ = p < 0.001, Student’s t test, n = 8.

(C) Representative actograms from C57Bl/6 mice injected with either Sik1 siRNA (siSik1, 50% knockdown of Sik1) or nontargeting siRNA (siNT). 4 days after injection of siRNA into the 3V the animals were maintained in constant dark in order for their free-running period to be determined.

(D) Mice treated with siSik1 show greater period length than control mice injected with siNT (p = 0.016, Student’s t test, n = 11, error bars = SEM).