Fig. 2.

Cytotoxic effect of CCCP. (A) SH-SY5Y cells were exposed with 10 μM CCCP, with vehicle (0.05% (v/v) ethanol) or without any treatment (control) for 24 h, and labeled with Ho to measure the chromatin condensation. Graphic shows percentages of cells with chromatin condensation (#p > 0.05; **p ≤ 0.01). The arrows highlight nuclei with chromatin condensation. (B) SH-SY5Y cells were exposed with 10 μM CCCP, with vehicle (0.05% (v/v) ethanol) or without any treatment (control) for 24 h, harvested by trypsinization and labeled with PI to measure cell viability by flow cytometry. Percentages of PI-positive cells (#p > 0.05; **p ≤ 0.01) and representative single-parameter histogram of PI measurement. Data were expressed as mean ± SEM; events = 10,000.