Table 1.

Proportion of cells with double staining in the skin and lung tissue^*

Tissue, sample	Protein of interest	CD31	CD68	α-SMA
Skin
Control	P-selectin	9.9 ± 3.2	4.5 ± 0.1	9.7 ± 4.8
SSc	P-selectin	49.2 ± 10.8†	51.2 ± 8.8†	27.6 ± 3.8†
Lung
Control	P-selectin	20.8 ± 15.0	26.5 ± 2.0	5.4 ± 4.0
SSc	P-selectin	42.0 ± 8.3	58.1 ± 7.2†	63.9 ± 6.6†
IPF	P-selectin	56.4 ± 7.4†	69.2 ± 3.2†	38.4 ± 6.4†
Lung
Control	CCL2	22.9 ± 3.6	20.6 ± 5.3	13.6 ± 4.9
SSc	CCL2	58.5 ± 6.5†	48.5 ± 6.8†	50.6 ± 9.7†
IPF	CCL2	65.7 ± 3.8†	59.9 ± 7.2†	60.9 ± 4.4†

^*Values are the mean ± SD proportion of cells with double staining (protein of interest plus marker protein). Proportions were determined by calculating the ratio of double-positive cells (i.e., P-selectin or CCL2 plus CD31 or CD68 or α-smooth muscle actin [α-SMA]) to single-positive cells (i.e., CD31 or CD68 or α-SMA only) on 4 representative images. SSc = systemic sclerosis; IPF = idiopathic pulmonary fibrosis.

^†P < 0.05 versus control subjects.