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. Author manuscript; available in PMC: 2014 Jan 22.
Published in final edited form as: J Invest Dermatol. 2012 Nov 29;133(5):1286–1293. doi: 10.1038/jid.2012.400

Figure 3.

Figure 3

SODD is a target of miR-26a. (a) Immunoblot. Cells were transfected with miR-26a mimic (26a) or Negative Control (c.) for 72 h. Quantifications are the ratios of the proteins to tubulin with controls set to 1.0. (b) Immunoblot. Cells were transfected with 50 nM miR-26a inhibitor (26aI) or miScript Inhibitor Negative Control (c.). (c) Dual-luciferase reporter assay. Luciferase reporter vectors containing SODD 3′UTR (Clones 1 and 2) or empty vectors were used. Relative luciferase activity was significantly decreased with miR-26a cotransfection (0.74 and 0.68, p = 0.002 and 0.0013, respectively) compared to control mimic for the constructs containing the SODD 3′UTR, but not for the empty vector (1.02, p = 0.82). Error bars represent SD of 3 replicates. *, p-value < 0.05.