Fig. 3.
Specificity of immune responses to antigen 85A. Parenterally or i.n. immunized BALB/c mice were sacrificed 4 weeks after the last immunization and the proportion of lung and spleen CD4 and CD8 lymphocytes producing IFNγ determined by intracellular cytokine staining after 6 h stimulation with the 85A99–118, 85A70–78 and 85A145–152 peptides. (A) Ad85A was administered i.n. or i.m. without adjuvant. (B) r85A was administered i.n. with cholera toxin or s.c. with MPL. (C) p85A were administered i.n. with cholera toxin or s.c. with MPL. Cells from 3 to 4 mice were assayed separately in at least 5 experiments. Error bars show SD.