Fig. 5.
Activation status of antigen specific CD4 and CD8T cells. Mice were sacrificed 4 weeks after the last i.n. immunization with Ad85A, r85A or p85A and after 6 h stimulation of lung and spleen with 15mer peptides covering the 85A99–118, 85A70–78 and 85A145–152 sequences, the activation status of CD8T cells producing IFNγ was determined by staining with CD62L and CD127 (A and B) and that of CD4T cells producing IL-2 by staining with CD62L and CD44 (C and D). The proportions of central (CD62L+ CD127+) or effector (CD62L− CD127+) memory or effector (CD62L− CD127−) antigen specific CD8T cells after Ad85A and p85A immunization are shown for lung (A) and spleen (B). Proportions of lung and spleen central (CD62L+ CD44lo) and effector (CD62L− CD44hi) memory antigen specific CD4T cells after different i.n. immunizations are shown in (C) and (D), respectively. Cells from 4 mice were assayed separately. Error bars show SD. Representative data from one of three experiments are shown.